Ot1 mouse
Web003831 C57BL/6-Tg(TcraTcrb)1100Mjb/J These mice contain transgenic inserts for mouse Tcra-V2 and Tcrb-V5 genes. The transgenic T cell receptor was designed to recognize ovalbumin peptide residues 257-264 (OVA 257 … WebThese transgenic mice are useful for studying in vivo T cell biology such as TCR-ligand interactions, T cell activation, thymic selection, cross-presentation of antigens, process of …
Ot1 mouse
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WebImmuno-Oncology Pharmacodynamic Models. Pharmacodynamic (PD) models allow the function of a therapeutic to be examined outside of the disease model. Following … WebJan 25, 2024 · Because OT1 mice share the C57BL/6J background, which is the background that most genetically modified mouse lines are developed, the OT1 mouse approach may provide a promising platform for further revealing the mechanisms by which Chlamydia-specific CD8 + T lymphocytes promote chlamydial pathogenicity in the female upper …
WebDec 14, 2015 · Here is my protocol: I mix at different effector to stimulator ratios (1:5, 1:1, 5:1) irradiated Act-OVA spleenocytes (serving as stimulators) and naive OT-1 … WebFeb 8, 2024 · (B) OT1 T cells were adoptively transferred into naïve mice that were anti-CD8 treated the next day. The following day the mice were immunized and the OT1 T cells FACS isolated from pooled lymph nodes and spleens 7 days later. OT1 T cells were then plated at an effector to target ratio of 1:1 with at least 3 technical replicates per treatment ...
Webwe demonstrate 13-color immunophenotyping of mouse splenocytes using the MACSQuant® Analyzer 16, a compact and reliable benchtop flow cytometer equipped with three lasers that combines the ease of use of previous MACSQuant Analyzer instruments with the option for high content analysis. The markers selected allow for the WebMar 9, 2005 · Mice. C57BL/6J(B6) female mice were purchased from Charles River Breeding Laboratories. RAG1 –/– mice, OT1 TCR transgenic mice, and OT2 TCR transgenic mice were purchased from The Jackson Laboratory. RAG1/OT2 Tg mice were generated by breeding OT2 Tg mice with RAG1 –/– mice. All mice were housed in the California Institute of …
WebJan 25, 2024 · OT1 mice failed to develop hydrosalpinx partially due to the failure of their lymphocytes to recognize chlamydial antigens. CD8 + T cells from naive C57BL/6J mice rescued the ability of recipient OT1 mice to develop hydrosalpinx when naive CD8 + T cells were transferred at the time of infection with Chlamydia.
WebPopular answers (1) 12th Feb, 2015. Paula M Chilton. University of Louisville. Phenotypically, these mice are the same as their homozygous siblings. Progression through T cell … joe henley rough \\u0026 gemstonesWebMouse models are commonly used to study various in vivo immune responses. Mouse MHC Tetramers can detect murine antigen-specific CTL. MBL conducted a study showing that antigen-specific CTL can be induced rapidly and easily at low cost using the following method. First, the antigen peptide of interest was mixed with a second “helper ... integration server software for enterprisesWebBestProtocols: RBC Lysis of Mouse Splenocytes protocol to remove red cells. 2. Count cells and resuspend in complete RPMI-1640 at 106/mL. Note: This density is optimal for TCR-mediated T cell activation in our experiments. A titration of cell densities (2-3x106 cells/mL to 105 cells/mL) is recommended for optimal activation in your studies. 3. joe hendry impact wrestling