Optical fan for single-cell screening
WebAug 5, 2024 · Search worldwide, life-sciences literature Search. Advanced Search Coronavirus articles and preprints Search examples: "breast cancer" Smith J WebFeb 15, 2024 · Single-molecule fluorescence in situ hybridization: a method where several fluorescent oligonucleotides are bound to individual DNA or RNA molecules, allowing them to be identified and counted...
Optical fan for single-cell screening
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WebThe single-cell screening platform was successfully fabricated by replicating each PDMS layer (the top control layer, the middle control layer, and the bottom cell culture/analysis layer) from the mater molds and assembling all the layers together (SI Fig. S1A-C). WebFeb 10, 2024 · The use of sophisticated models such as organoids and whole organisms, flexible perturbations such as gene activation and repression, diverse biological challenges and data-rich read-outs such as...
Webcell line.19 Imaging flow cytometry has succeeded by combining the optical characterization of cell size and morphology,9 ,2024 refractive index,1112 or deformability,22−24 with proven capability for label-free cell screening in leukemia diagnosis.25 A more recent route in label-free cytometry proposes the use of mechanical …
WebJan 19, 2024 · An optical approach for cell enrichment by patterned illumination followed by FACS sorting We developed an approach, which we term optical enrichment, to select cells of interest using a microscope and mark them by photoactivation, enabling cell isolation using FACS ( Fig. 1 ). WebJul 18, 2024 · In this work, by using a specific‐designed fiber probe with a flat facet, we propose an “optical fan” strategy to screen K562 cells at the single‐cell level from a populations of RBCs....
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WebDec 23, 2024 · An artificial test mixture of green-fluorescent protein (GFP) E. coli, non-GFP E. coli and yeast were loaded onto the chip in a 1:1:1 ratio, and quickly the GFP bacteria and yeast were separated ... cincinnati airport drop offWebfluidic chip, an optical time-stretch quantitative phase microscope, and a digital image processor equipped with machine learning. As a result, it provides both the opacity and phase maps of every single cell at a high throughput of 10,000 cells/s, enabling accurate cell classification without the need for fluorescent labeling. dhr hospital numberWebJan 2, 2024 · An optical fan was demonstrated to screen leukemia cells from the blood sample at the single-cell level in a noninvasive and noncontact manner. Further details can be found in the article by Xiaoshuai Liu, Yuchao Li, … cincinnati airport flights to ft myersWebDec 23, 2024 · December 23, 2024 Optical 'tweezer' enables fast, low-cost screening of bacteria and cancer cells by Li Yuan, Chinese Academy of Sciences Versatile, facile and low-cost single-cell... dhr hospital recordsWebFeb 1, 2024 · CRISPR (clustered regularly interspaced short palindromic repeats)-based gene inactivation provides a powerful means for linking genes to particular cellular phenotypes. CRISPR-based screening typically uses large genomic pools of single guide RNAs (sgRNAs). However, this approach is limited to phen … dhr hoursWebLiu, X., Li, Y., Xu, X., Zhang, Y., & Li, B. (2024). Optical fan for single‐cell screening. Journal of Biophotonics. doi:10.1002/jbio.202400155 cincinnati airport cheap flights to floridaWebNov 29, 2024 · The target cell is then captured by a 1064 nm optical tweezer and deposited as one-cell-harboring nanoliter microdroplets in a one-cell-one-tube manner. For bacterial, yeast and human cells, OPSI achieves a >99.7% target-cell sorting purity and a 10-fold elevated speed of 10–20 cells per min. cincinnati airport covid testing