K1622 thermo scientific
Webb(K1622) RevertAid First Strand cDNA Synthesis Kit - Thermo Scientific - CiteAb (K1622) RevertAid First Strand cDNA Synthesis Kit Sign in or register to save this reagent to … WebbDescription RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb. RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA templates from degradation.
K1622 thermo scientific
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Webb8 mars 2024 · Cells in each group were digested and collected with trypsin (15050065, Gibco, Thermo Fisher Scientific Inc.) without EDTA. Then, the cells were resuspended with 100 μL binding buffer after washing with phosphate buffer solution (PBS), and 3 μL Annexin V and 3 μL PI (Annexin V-FITC staining kit, Beyotime Biotechnology Co. Ltd., … WebbFirst-strand cDNA was synthesized from total RNAs using RevertAid First Strand cDNA Synthesis kit (#K1622; Thermo Fisher Scientific, Waltham, MA, USA). Quantitative PCR was performed using iQ SYBR Green Supermix (#170882; Bio-Rad, Hercules, CA, USA) according to the manufacturer’s recommendations.
Webbบริษัท ออโตมอร์ จำกัด ๕๑/๘๔๙ หมู่ที่ ๒ ซ.ลำลูกกา๑๓ ถ.ลำลูกกา ต.คูคต อ.ลำลูกกา จ.ปทุมธานี ๑๒๑๓๐ โทรศัพท์ ๐-๒๙๙๕-๕๕๗๒ โทรสาร ๐-๒๙๙๕-๕๕๗๓ Webb25 apr. 2016 · K1622产品描述. Thermo Scientific RevertAid*链cDNA合成试剂盒包含了足够进行100次x20 μL反应的试剂。本品是以RNA为模板高效合成*链cDNA的完整系统,可合成长达13kb的cDNA。本品含有RiboLock RNase抑制剂,能够有效保护RNA模板不受降解。 Thermo Scientific RevertAid*链cDNA合成试剂盒 ...
Webb10 apr. 2024 · Total RNA was extracted using Trizol reagent (Invitrogen), then reverse transcribed into cDNA using a RevertAid First Strand cDNA Synthesis Kit (cat. no. K1622, Thermo Fisher Scientific, USA). Webb11 apr. 2024 · The complementary DNA synthesis was performed using the kit manufacturer’s protocol (K1622: Thermo Scientific ®, Germany). For quantification and amplification, the SYBR Select Master Mix kit (Applied Biosystems Thermo Scientific ®, Germany) was used on qRT-PCR (Applied Biosystems Thermo Scientific ®).
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WebbQuick Order. Order Status and Tracking. Documents and Certificates. Search All. Order Status. Quick Order. Sign in. Error: This product (Catalog Number K1222) is not … sharlene wattsWebb22 sep. 2016 · The cDNA was reverse-transcribed using the RevertAid First-Strand cDNA Synthesis kit (cat. no. K1622; Thermo Fisher Scientific, Inc.), and RT-qPCR experiments were performed using the SYBR Premix Ex Taq™ II kit (Takara Biotechnology Co., Ltd., Dalian, China) and cycled at 95°C for 10 min followed by 39 cycles of 95°C for 15 sec … sharlene weaver-greaves chicago entainerWebbThermo Scientific CloneJET PCR Cloning Kit #K1232 40 rxns Lot Expiry Date Store at -20 °C www.thermoscientific.com/onebio COMPONENTS OF THE KIT CloneJET PCR … sharlene watson np