WebThe Transgenic and Knockout Mouse Shared Resource provides members of the DCI with comprehensive and user-friendly services for the production of custom designed, … WebInterferon regulatory factor (IRF) 8 is a transcriptional regulator that is essential for the differentiation and function of monocytes and macrophages, and thus may be involved in the regulation...

Frontiers IRF8-Dependent Type I Conventional Dendritic Cells …

WebDec 11, 2024 · Mice with IRF8 deficiency only in colon epithelial cells (termed IRF8cKO) were created by crossing Irf8-floxed mice with villin-cre mice (Figures S1A and S1B) with the deletion of exon 2 (Figure S1C). As reported in the literature, we observed that IRF8KO mice exhibit elevated levels of CD11b + Gr1 + MDSCs as compared to wild-type (WT) mice. … sims cc stockings

IRF8 is involved in cutaneous wound healing in mice and patients.

WebMice in which the gene expressing B 2 receptor has been specifically deleted from the endothelium (B 2 flox/flox.Tie2 Cre) presented normal blood pressure readings compared … WebFeb 1, 2011 · IRF8-null mice (IRF8 −/ ... Fig. 3A illustrates the strategy for conditional deletion of exon 2 of Irf8. The floxed and WT alleles were detected by PCR in genomic DNA . After crossing with CD19-Cre mice, the homozygous Irf8 f/f mice bearing the Cre gene exhibited a complete depletion of IRF8 protein in splenic B cells . In the BM ... WebMyeloid cell-specific IRF8 conditional knockout mice (IRF8flox/flox; Lyz2Cre/+) with a C57BL/6 background were generated by mating IRF8-flox mice (Feng et al. 2011) with Lyz2-Cre knock-in mice. The primers used for genotyping are shown in Supplementary Table S3. The genotyping results are shown in Supplementary Figure S4a. Harvesting of blood ... sims cc steam