Huh7 and Huh7.5
WebOct 9, 2024 · The human hepatoma-derived HuH-7 cell line and its derivatives (Huh7.5 and Huh7.5.1) have been widely used as a convenient experimental substitute for primary hepatocytes. In particular, these... WebJan 31, 2024 · Twelve mice bearing the tumors generated from chemotherapy‐damaged HCC cells Huh7 (2 × 10 7) with endothelial cells EA.hy926 (4 × 10 5) were divided into two groups randomly and treated with either DMSO (control group, n = 6) or thalidomide (50 mg/kg, three times a week, n = 6) intraperitoneally. After 4 weeks, animals were …
Huh7 and Huh7.5
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Webelectroporation protocols yielded 63.73 ± 2.36 and 73.9 ± 1.6% of transfection in Huh7 and Vero cells respectively, while maximum achieved level of transfection by jetPEITM was 14.2 ± 0.69 and 28 ± 1.11% Huh7 and Vero cells, respectively. Post transfectional chilling of the cells did not improve electrotransfection efficiency of Huh7 cells. WebThen, the viral supernatant was replaced with fresh media. After 5 days of infection, the knockdown efficiency was determined by RT-PCR and western blot. 14 Huh7 and SMMC-7721 parental cells, control transfectants, and COX-2 transfectants were named Huh7-P, Huh7-C, Huh7-S, SMMC-7721-P, SMMC-7721-C, and SMMC-7721-S cells, respectively.
WebFeb 22, 2010 · Importantly, D183-infected Huh7 and Huh7.5.1 cells also induced IFNα ( Fig. 1 C) and IFNβ production ( Fig. S1 D) by pDCs, as did Huh7.5.1c2 cells infected by JFH1/D18 (D18) virus ( 15) and by chimeric HCV strains ( 17) based on JFH1 ( Fig. 1 D and Fig. S1 E ). Fig. 1. IFNα production by coculture of pDCs and HCV-infected cells. WebHuH-7 Cells should be cultured in DMEM supplemented with 10% FBS. Cells should be grown at 37 °C in a 5% CO2 setting. Subculture Protocol for HuH-7 To subculture: Renew medium 2-3 times a week, or as needed according to media pH, and maintain cell confluency between 30-90%.
WebHuh7 (4×10 5) seeded cells were incubated with 20 mg/mL of EV-Huh7/NIS for 24 hours. The cells were washed with bHBSS and incubated with or without 50 μCi/mL 131 I … WebDec 19, 2024 · The innate immunity response of Huh7.5 cells, a derivative clone of Huh7 cells, was previously shown to be more impaired than that of Huh7 cells when both cells …
WebJan 27, 2024 · The HuH7.5 cell line is a derivative of the HuH7 cell line. Unlike its parental cell line, HuH7, it possesses a missense mutation in the RIG-I or DDX58 gene, which is …
WebThe simulation results of activity levels of 10 signaling proteins in the Huh7 cell line under various perturbations, in comparison with experimental results. (XLSX) Comparative … south west regional college of tafe bunburyWebApr 29, 2005 · Huh7.5, a Huh7 subline that is highly permissive for HCV RNA replication , has a lethal mutation in the RIG-I CARD-like homology domain that renders it unresponsive to structured HCV RNA or SenV induced signaling . Whereas this accounts for the highly permissive phenotype of Huh7.5 cells, it does not fully explain why parental Huh7 cells … teamdealer freeWebApr 12, 2024 · Mirroring the partial recovery of ALIX recruitment in Huh7 ATG3/5-DKO relative to Huh7 ATG5-KO cells in response to lysosomal damage, Gal3 as a marker of … southwest regional council of nahroWebHuH-7.5. HuH-7 is a well differentiated hepatocyte derived cellular carcinoma cell line that was originally taken from a liver tumor in a 57-year-old Japanese male in 1982. The line … south west regional conservatoriumsouth west regional divorce centrehttp://yuxiqbs.cqvip.com/Qikan/Article/Detail?id=43743942 southwest refunds on flightsWebFeb 19, 2010 · The clone Huh7.5 of human hepatoma Huh7 cells contains a mutation in RIG-I that is believed to be responsible for the improved replication of HCV in these cells … southwest regional airport washington