Fitc dmso

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August undefined, 2024

WebFITC (fluorescein-5-isothiocyanate) is an amine-reactive fluorescein derivative for labeling proteins or other molecules containing primary amines. λ Ex /λ Em = 492/520 nm. ε = 75,000. Orange solid soluble in DMF or DMSO. Store at -20°C and protect from light, especially in solution. http://www.rx-8.com/chanpinzhongxin/206641.html

FITC-labelled polysaccharides - Sigma-Aldrich

Web1.8 Mg of PU-FITC in an amber Eppendorf tube was dissolved in 200 μL of DMSO [molecular biology grade from Sigma Aldrich] and vortexed to make 10 mM PU-FITC solution. This 10 mM PU-FITC solution was further diluted (10-fold) with DMSO to make 2 mL of 1 mM PU-FITC solution and stored as small aliquots of 50 μL in 40 amber … http://vp-sci.com/media/productattachments/files/2/6/267a_pin_tool_volume_fitc_protocol.pdf razertip ss-d10 dual burner

FITC (Fluorescein 5-isothiocyanate) Fluorescent Dye

WebAug 24, 2007 · Hello, you scientists ! I got a problem on FITC preparation . Because I'd like to add peptide-FITC mixture into bacteria and animal cell. And according to Sigma's … WebFITC is soluble in DMSO and DMF. Applications. General. FITC is commonly used for labeling antibodies 2 (IgG) and also in other immunological applications. It is also often used in flow cytometry, a technology frequently used by biologists to study cellular populations with high precision 3. WebAug 5, 2013 · The FCS study reveals a change of the hydrodynamic radius of BSA from 3.7 nm in the native state to 7.0 nm in the presence of 40% DMSO, which suggests complete unfolding of the protein under these conditions. Fluorescence self-quenching of FITC has been exploited to understand the conformational dynamics of BSA. simpson on the strand menu

Fluorescein Isothiocyanate - an overview ScienceDirect Topics

Fluorescein isothiocyanate - Wikipedia

WebFluorescein isothiocyanate ( FITC) is a derivative of fluorescein used in wide-ranging applications [1] [2] including flow cytometry. First described in 1942, [3] FITC is the … Web瑞禧生物提供科研级FITC-PEG(3)-NH2,我们一直致力于研发纳米靶向科研产品以满足国内广大科研院校及其他相关科研单位的需求。 ... Fluorescein-PEG3-Amine 外观：Liquid 分子量：581.638 溶解度：DMSO CAS号：1807539-04-3 质量控制：95% 储存条件：-20 ... simpson optical - meijer wayWebProtocols of Sigma and MPBio are the same. They suggest to prepare protein solution not less than 2 mg/ml and add 50 ul of FITC (1 mg/ml in … razertip wood burner uk

"WebB. Preparing FITC Dilutions Dissolve the FITC (250 mg/ bottle) in 4 mL of DMSO, by pipetting the DMSO into the container. Leave the container in a dark place at room temperature for 2 hours (or until all the FITC has dissolved.) Pour the entire dilution into a 500 mL VWR bottle. Add enough DMSO to fill the bottle to the 500 mL mark. " - Fitc dmso

Fitc dmso

Stearic acid PEG Fluorescein，FITC-STA-PEG，硬脂酸聚乙二醇荧光素，一种具有末端荧光FITC …

WebApr 7, 2024 · 硬脂酸-PEG荧光素（Stearnic acid PEG-FITC）是一种具有末端荧光FITC的两亲性表面活性剂。. 硬脂酸是一种18碳饱和脂肪酸脂质，具有优异的疏水性，PEG修饰的硬脂酸是一种具有亲水性和疏水性的优良的两亲性聚合物。. 硬脂酸是十八烷酸CH3（CH2）16COOH的另一个名称，是 ... WebNov 22, 2011 · In vitro disease modeling using pluripotent stem cells can be a fast track screening tool for toxicological testing of candidate drug molecules. Dimethyl sulfoxide (DMSO) is one of the most commonly used solvents in drug screening. In the present investigation, we exposed 14- to 21-day-old embryoid bodies (EBs) to three different …

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WebASHBURN (HQ) 44320 Premier plaza, Suite 210, Ashburn, VA 20147 Phone: 1-703-263-0855 Email: [email protected] WebFITC is tested for solubility and solution appearance at 1 mg/ml in acetone. It is soluble in anhydrous dimethyl sulfoxide (DMSO) at 5 mg/ml.10 It is soluble in water at less than 0.1 …

WebFITC-xtra conjugates are significantly brighter than FITC conjugates and are much more photostable. Additionally, the fluorescence of FITC-xtra is not affected by pH (4-10). Cat No. 135 ... DMSO: Spectral properties. Excitation (nm) 498: Emission (nm) 526: Storage, safety and handling. H-phrase: H303, H313, H333: Hazard symbol: XN: Intended use ... Web西安齐岳生物科技有限公司生产销售“四臂聚乙二醇荧光素 4ARM-PEG-FITC”-“中文名称四臂聚乙二醇荧光素四臂PEG荧光素英文名称 4ARM-PEG-FITC 4Arm-PEG-Fluoresein 溶解度溶于DMSO DMF DCM等有机溶剂，溶于水”，该产品仅用于科研，如果需要请联系我们 .

WebApr 11, 2024 · 中文名称：荧光素-聚乙二醇-双棕榈酸甘油酯英文名称：fitc-peg-dpg 外观：取决于分子量溶解性：water, dmso, dcm 纯度：95% 活性基团：fitc 分子量：400 600 1000 2000 3400 4000 5000 10000 20000 注意事项取用保持低温干燥，现配现用，避免频繁冻融造成产品失活存储条件 -20 ... WebDMSO: 5 mg/mL DMF: soluble aqueous buffer, pH ≥ 9.0: soluble. storage temp. 2-8°C. ... various proteins, and oligopeptides with fluorescein. FITC-antibody conjugates are useful in many applications, including flow cytometry and immunohistochemistry (excitation maximum = 492 nm; emission maximum = 520 nm). λ max: 492 ± 3 nm (pH 9 ...

WebFITC (Fluorescein isothiocyanate) is the form of fluorescein used for conjugation to all JIR antibodies and purified proteins, with the exception of streptavidin. Fluorescein …

WebFITC: fluorescein isothiocyanate; used as a fluorescent label for proteins, especially antibodies. simpson optical - tates creekThen, use anhydrous DMSO to dissolve FITC at 1 mg/ml. Do not use stored solutions of FITC in DMSO; prepare fresh on the day of the reaction. Step 2. Incubate Protein with FITC. Slowly and gently add 50 ul of FITC solution to each ml of protein solution in 5 ul intervals while stirring the mixture. Incubate the solutions in the dark at 4 °C for ... razertip wood burner pensWebDec 11, 2024 · Dissolve FITC in anhydrous DMSO at 1 mg/ml concentration. This should be prepared fresh for each labelling reaction. Add 5 μl of the dye to the dialysed protein (5 … simpson optical and medicaidWebFor example, using 5 mg of IgG and a 10∶1 molar ratio of fluorescein isothiocyanate (FITC) with mol wt of 389: (2) 3. Prepare a 10 mg/mL solution of the fluorophore by weighing 3 or more mg of dye and dissolving it in 0.3 mL or more of DMF or DMSO. Sulfonyl ... DMSO, or ethanol. Dilute this solution approx 1∶1000 in the reaction mixture to ... razertip wood burning toolsWebAug 1, 2024 · For DMSO/DBTD method, the fluorescent label is tagged to the hydroxyl group of AX. FITC can be grafted onto AX through thiocarbamoyl linkage, as shown in Fig. 1 a. FITC reacts with AX in DMSO at elevated temperature to give O-(fluoresceinyl-thiocarbamoyl) AX with pyridine and DBTD as the catalysts. During the reaction, pyridine … simpson optometryWebFITC, 1g . FITC, 100mg . Chemical name: Fluorescein isothiocyanate: Molecular weight: 389.38 . Extinction coefficient: 70,000M-1: cm-1 ... Dissolve TRITC in DMSO at 1mg/mL. 2. While stirring, slowly add 35µl of TRITC to 1mL of the 6mg/mL antibody solution and mix thoroughly. 2. Incubate for 2 hours at room temperature in the dark. simpson optical meijer wayWebAug 1, 2011 · Preparation of fluorescein labeled SA (FI-SA) One gram of the SA dissolved in 10 mL of DMSO was reacted with 25 mg of FITC in the presence of 1.5 mL DBTDL for 5 … razer titans headphones