WebCy3 Dyes. Cy®3 is an orange fluorophore commonly used in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. The dye has an absorption wavelength that peaks around 548-552 nm, and an emission maximum around 562-570 nm. Cy®3 is bright, photostable, and can be used as a … Webprotein and Green fluorescent proteins in close enough proximity to allow for FRET to occur. In this example, excitation of blue fluorescent protein results in the emission of fluorescence by Green fluorescent protein. The organic cyanine dyes Cy3, Cy5, Cy5.5 and Cy7, which emit in the red range (>550 nm), offer a number of advantages.

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Web4）Q：Cy3与Cy5有什么区别？ A：荧光基团不同，检测时发射荧光的颜色不同（Cy3 azide最大激发波长555 nm， 最大发射波长570 nm；Cy5 azide最大激发波长646 nm，最大激发波长662 nm），两者的实验效果相同，可根据个人需求进行选择。 5）Q：样本检测不到EdU阳性信号？ WebEx vivo fluorescent imaging showed a strong signal in tumors, heart, liver, and kidney 4 h after injection, while a fluorescent signal was detected in tumors but not other organs 16 h after injection ... Cy3-labeled USP14 siRNA fluorescent images were obtained using the Caliper IVIS Lumina III (Perkin Elmer, USA) at 5, 10, ... common marine workshop safety rules

Atto Dyes for Superior Fluorescent Imaging - Sigma-Aldrich

WebSep 20, 2007 · The sulfoindocyanine Cy3 is one of the most commonly used fluorescent dyes in the investigation of the structure and dynamics of nucleic acids by means of … WebThe fluorescent properties of cyanine dyes are known to be sequence-dependent, with purines in the immediate vicinity increasing the fluorescence intensity of Cy3 and Cy5 dyes, and the ability of ... WebFluorescent labeling of the thiol group of cysteines is preferred for investigating structure, function and interactions of the POI. (Please note: Jena Bioscience offers a ready-to-use … commonmark code block