Chimeric lysin
WebSep 15, 2014 · In the present work, a novel chimeric lysin (Ply187N-V12C) was constructed by fusing Ply187N with the CBD (146-314aa, V12C) of PlyV12 to test if the lytic specificity of Ply187N would be extended to … WebNov 4, 2013 · The chimeric lysin ClyH was constructed by fusing the N-terminal 157 amino acids of Ply187 (Pc) with the C-terminal 97 amino acids of phiNM3 lysin. To do this, the DNA fragment encoding the chimeric lysin was chemically synthesized by Songon Biotech (Shanghai, China).
Chimeric lysin
Did you know?
WebJun 3, 2024 · Developing chimeric lysins with a wide lytic spectrum is important in fighting against Gram-negative pathogenic bacteria. In the present work, a novel chimerical lysin, LytAmfi, was constructed by fusing the bacteriophage endolysin Lyt μ1/6 with an amphipathic cationic peptide derived from T4 lysozyme. The result showed that the … WebThe chimeric lysin, ClyC, was expressed as described previously (Li et al., 2024). Concentration of ClyC was determined by BCA assay and visualized by SDS-PAGE. The ClyC protein solution was freeze-dried and then …
WebNov 16, 2024 · Cationic peptide chimeric lysins, Lysqdvp001-5aa, Lysqdvp001-10aa and Lysqdvp001-15aa, were designed based on lysin Lysqdvp001 from Vibrio … WebJan 9, 2024 · Developing chimeric lysins (chimeolysins) by shuffling these two domains from different natural lysins has been demonstrated to be a …
WebLysin definition, an antibody causing the disintegration of erythrocytes or bacterial cells. See more. WebMar 27, 2024 · In addition, the chimeric lysin, ClyJ, was constructed by fusing GPB coding sequence to the C terminus of the CHAP domain of PlyCA (amino acids 314 to 465) using sequence-specific primers (Table S4). The pneumococcal Cpl-1 lysin coding sequence was chemically synthesized by GenScript (Nanjing, China) and cloned into a pET28b(+) …
WebMar 1, 2015 · The increased lethal action of Cpl-711 has been validated in a mouse model of bacteraemia, confirming that this new chimeric lysin is the most potent enzyme against pneumococci described so far. Materials and methods Ethics statement. All experiments were performed under strict adherence to the NIH guidelines for the ethical treatment of …
WebJan 9, 2024 · Yang, H. et al. Novel chimeric lysin with high-level antimicrobial activity against methicillin-resistant Staphylococcus aureus in vitro and in vivo. Antimicrob. Agents Chemother. 58 , 536–542 ... greatest hits on mtvWebApr 19, 2024 · In this study, a novel chimeric lysin, ClyC, was constructed by fusing the CD from the Ply187 lysin with the CBD from the LysSA97 lysin. The CD of Ply187 … greatest hits - pam tillisWebApr 19, 2024 · In this study, a novel chimeric lysin, ClyC, was constructed by fusing the CD from the Ply187 lysin with the CBD from the LysSA97 lysin. The CD of Ply187 (1–157aa, Ply187N) was chosen because it has been shown to have much higher activity compared to its parental full length S. aureus phage Ply187 [ 25 ]. greatest hits part 1WebMar 27, 2024 · Based on the CW_7 repeats, chimeric lysins engineered by domain swapping between Cpl-7 and other lysins demonstrated increased bactericidal activities … greatest hits pdfWebSH3b-like CBD of phiNM3 lysin (Yang et al., 2014), the chimeric lysins showed even higher lytic activity than Ply187N. In the present work, a novel chimeric lysin (Ply187N-V12C) was constructed by fusing Ply187N with the CBD (146-314aa, V12C) of PlyV12 to test if the lytic specificity of Ply187N would be extended to other genera by adding flipped book quotesWebAug 24, 2024 · Lysine is an herbal supplement used for athletic performance enhancement, or treatment for cold sores (herpes simplex libialis) and elevated pH (metabolic … flipped book quotes with page numbersWebFeb 10, 2024 · The control was performed without chimeric lysin. (A) Changes in total viable counts in the chilled chicken during storage at 4 °C for 14 days. (B) Changes in the pH of the chilled chicken during ... greatest hits part i