Bulked segregant analysis bsa dna sequencing
WebJan 28, 2024 · Key message Using bulked segregant analysis of exome sequence, we fine-mapped the ABA-hypersensitive mutant ERA8 in a wheat backcross population to … WebApr 13, 2024 · Here, bulked segregant RNA-Seq was used to identify two candidate genes for melanoid, which, in comparison to the wild type, is characterized by increased …
Bulked segregant analysis bsa dna sequencing
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WebBulked segregant analysis (BSA) has been used to map many agronomical traits in several crops, including resistance genes to fungal pathogens. Using BSA in diploid … WebPericarp color in wheat is an important trait related to nutrients and health benefits. Identification of molecular markers closely linked to the grain color g
WebSequencing this stretch of DNA identified the central portion as the putative Mat1-2 locus of M graminicola. ... NGS-based mapping approaches using bulked segregant analysis … WebNov 3, 2011 · We describe a statistical framework for QTL mapping using bulk segregant analysis (BSA) based on high throughput, short-read sequencing. Our proposed …
WebBulked segregant analysis ( BSA) is a technique used to identify genetic markers associated with a mutant phenotype. This allows geneticists to discover genes conferring … WebNov 25, 2024 · Therefore, this study aimed to identify significant QTLs associated with NCLB by utilizing next-generation sequencing-based bulked-segregant analysis (BSA). Parental lines GML71 (resistant) and Gui A10341 (susceptible) were used to develop segregating population F 2. Two bulks with 30 plants each were further selected from the …
WebDec 21, 2024 · Bulk segregation analysis (BSA) utilizes a strategy of pooling individuals with extreme phenotypes to conduct economical and rapidly linked marker screening or quantitative trait locus (QTL) mapping. With the development of next-generation sequencing (NGS) technology in the past 10 years, BSA methods and technical …
WebBulk segregation analysis (BSA) utilizes a strategy of pooling individuals with extreme phenotypes to conduct economical and rapidly linked marker screening or quantitative … ghw bush library and museum storeWebSep 27, 2024 · A combination of specific-locus amplified fragment sequencing (SLAF-seq) and bulked segregant analysis (BSA) was used to identify candidates for regions associated with anthocyanin accumulation. g h w bush vpWebMar 9, 2024 · 从f2群体中选择黄绿叶植株和绿叶植株构建极端表型的dna 混池，采用bsa分析发现，在第3号染色体短臂和长臂上各存在1个明显的Δsnp指数曲线的峰（图3），这两个区间可能存在控制gy157s 黄绿叶表型的候选基因。 frost fest 2022 reginaWebApr 14, 2024 · Bulked segregant RNA-seq (BSR-seq) couples BSA with RNA-seq (Liu et al., 2012) and can be used not only to identify differentially expressed genes (DEGs) but also single-nucleotide polymorphisms (SNPs) that are tightly linked to targeted traits (Du et al., 2024). BSR-seq has been successfully applied in analyses of many species. g h w bush socksWebJan 26, 2012 · Bulked segregant analysis (BSA) was used to define a subset of putative SNPs within the candidate gene region, which were then used to fine-map GPC-B1. Results: After discriminating for SNPs between the two homoeologous wheat genomes and additional quality filtering, we identified inter-varietal SNPs in wheat unigenes between … frost fest 2022 hays ksWebMay 7, 2012 · Bulked segregant analysis (BSA) is an efficient method to rapidly and efficiently map genes responsible for mutant phenotypes. BSA requires access to quantitative genetic markers that are polymorphic in the mapping population. ghwccWebJun 15, 2024 · DNA samples from two bulk DNA pools (L-pool and S-pool) and their parents (KJ01 and Huaye 3) were subjected to high-throughput sequencing. Using bulked segregant analysis (BSA), qGL3.5 was mapped to a 0.34-Mb “hotspot” region on chromosome 3 that contains 37 genes related to various traits. ghw collins.com